Local alignment

local alignemnt

Blast (Basic Local Alignment Search Tool )

Is an algorithm for comparing primary biological sequence information

Program Query Search Database
blastn DNA -> DNA
blastp protein -> protein
blastx DNA -> protein
  • Bit score : The higher the bit-score, the better the sequence similarity

  • Identity : $$identity = \frac{match}{total} $$

    ATCTG
|| ||   identity : 80%
ATATG

  • E-value : The smaller the E-value, the better the match.

$$Eval(S)= K M N * e^{-lambda*S}$$

Global alignment

global alignment

Minimap2